ABSTRACT Introduction: Cardiopulmonary capacity and sports endurance are very important for running athletes. Objective: Study whether running with load training can improve physical fitness, focusing on running athletes’ cardiopulmonary capacity and exercise endurance. Methods: 36 professional athletes were selected. One group was trained with normal running and the other group was trained with running with a weight load. Results: In the weight-bearing training group, FVC went from 5.13 ± 0.20 to 5.68 ± 1.25, FEV1 went from 4.38 ± 0.33 to 4.56 ± 0.35, PEF went from 8.27 ± 0.98 to 8.88 ± 1.02, ERV went from 1.44 ± 0.37 to 1.68 ± 0.34, IVC went from 4.91 ± 0.45 to 5.28 ± 1.46, VT went from 1.10 ± 0.25 to 1.42 ± 1.26, systolic blood pressure varied from 115.16 ± 8.18 to 117.02 ± 5.47, diastolic blood pressure varied from 78.58 ± 8.97 78.16 ± 7.42, BPM varied from 73.42 ± 9.27 to 73.52 ± 9.02, serum lactic acid was from 1.36 ± 0.28 to 1.31 ± 0.27, and serum creatine kinase was from 21.85 ± 59.02 to 208.11 ± 49.20. Conclusion: Load running training positively impacts athletes’ physical fitness and may be added to improve athletes’ cardiorespiratory capacity and exercise endurance. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMEN Introducción: La capacidad cardiopulmonar y la resistencia al deporte son muy importantes para los atletas que corren. Objetivo: Estudiar si correr con entrenamiento de carga puede mejorar la forma física, centrándose en la capacidad cardiopulmonar y la resistencia al ejercicio en atletas corredores. Métodos: Se seleccionaron 36 atletas profesionales. Un grupo fue entrenado con carrera normal y el otro con carrera con carga de peso. Resultados: En el grupo de entrenamiento con carga de peso, la FVC pasó de 5,13 ± 0,20 a 5,68 ± 1,25, el FEV1 pasó de 4,38 ± 0,33 a 4,56 ± 0,35, el PEF pasó de 8,27 ± 0. 98 a 8,88 ± 1,02, ERV pasó de 1,44 ± 0,37 a 1,68 ± 0,34, IVC pasó de 4,91 ± 0,45 a 5,28 ± 1,46, VT pasó de 1,10 ± 0,25 a 1,42 ± 1. 2645, la presión arterial sistólica varió de 115,16 ± 8,18 a 117,021 ± 5,47, PPM varió de 78,58 ± 8,97 a 78,16 ± 7,42, las pulsaciones por minuto variaron de 73,42 ± 9,27 a 73,52 ± 9,02, el valor de ácido láctico en sangre fue de 1,36 ± 0,28 a 1,31 ± 0,27, y la creatina quinasa sérica fue de 219,85 ± 59,02 a 208,11 ± 49,20. Conclusión: El entrenamiento de carrera con carga influye positivamente en la forma física de los atletas, y puede añadirse para mejorar la capacidad cardiorrespiratoria y la resistencia al ejercicio de los atletas. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

RESUMO Introdução: A capacidade cardiopulmonar e a resistência esportiva são muito importantes para os atletas de corrida. Objetivo: Estudar se o treinamento de corrida com carga pode melhorar a aptidão física, com enfoque na capacidade cardiopulmonar e a resistência ao exercício dos atletas de corrida. Métodos: Foram selecionados 36 atletas profissionais. Um grupo foi treinado com corrida normal e o outro grupo foi treinado com corrida com carga de peso. Resultados: No grupo de treinamento com rolamentos de peso, FVC passou de 5,13 ± 0.20 para 5,68 ± 1,25, FEV1 passou de 4,38 ± 0,33 para 4,56 ± 0,35, PEF passou de 8,27 ± 0,98 para 8,88 ± 1,02, ERV passou de 1,44 ± 0,37 para 1,68 ± 0,3431, IVC passou de 4,91 ± 0,45 para 5,28 ± 1,46, VT passou de 1,10 ± 0,25 para 1,42 ± 1,26, pressão arterial sistólica variou de 115,16 ± 8,18 para 117,02 ± 5,47, pressão arterial diastólica variou de 78,58 ± 8,97 78,16 ± 7,42, BPM variou de 73,42 ± 9,27 para 73,52 ± 9,02, o valor de ácido lático sanguíneo foi de 1,36 ± 0,28 para 1,31 ± 0,27, e a creatina quinase sérica foi de 219,85 ± 59,02 para 208,11 ± 49,20. Conclusão: O treinamento de corrida com carga impacta positivamente a aptidão física dos atletas, podendo ser acrescido para melhorar a capacidade cardiorrespiratória e a resistência ao exercício dos atletas. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMO Introdução: Exercícios podem retardar os efeitos do envelhecimento e melhorar a função física dos idosos. O Tai Chi é uma prática popular de exercício entre os idosos na China. Embora haja estudos que evidenciem os efeitos positivos da prática de Tai Chi, ainda não há um consenso sobre os estudos comparados. Objetivo: Avaliar o efeito do exercício regular de Tai Chi sobre a estabilidade neuromuscular nos membros inferiores em idosos, comparando os resultados dos praticantes entre grupos idosos de sedentários e praticantes de caminhada. Métodos: Foram selecionados vinte e dois praticantes de Tai Chi, com idade média de 59,3±3,5 anos com tempo médio de prática de 18,4±13,2 anos. Este experimento testa principalmente a capacidade de equilíbrio comparando aos indivíduos que praticam caminhada e outros sedentários. Resultados: A força muscular dos flexores e extensores do joelho no grupo Tai Chi foi significativamente maior do que no grupo sedentário (p=0,001 a 0,00160°/s; P=0,002 a extensores de 60°/s; p=0,002 a 120°/s; flexores de 120°/seg, p=0,003). Similarmente, houve uma diferença significativa na força muscular entre o grupo Tai Chi e o grupo de caminhada (os valores P dos flexores e extensores em ambas as velocidades foram inferiores a 0,001). Conclusão: Exercícios regulares, como o Tai Chi, podem alterar a força muscular dos flexores e extensores gerais do joelho, e melhorar a estabilidade neuromuscular dos idosos nos membros inferiores. Nível de evidência II; Estudos Terapêuticos - Investigação de Resultados.

ABSTRACT Introduction: Exercise can retard the effects of aging and improve the physical function of the elderly. Tai Chi is a widespread exercise practice among the elderly in China. Although studies show the positive effects of Tai Chi practice, there is no consensus about compared studies. Objective: Evaluate the impact of regular Tai Chi exercise on neuromuscular stability in the lower limbs of elderly people, comparing the results of practitioners between sedentary and walking elderly groups. Methods: Twenty-two Tai Chi practitioners were selected, with a mean age of 59.3±3.5 years and a mean practice time of 18.4±13.2 years. This experiment mainly tests the balance ability compared to individuals practicing walking and other sedentary individuals. Results: The muscle strength of the knee flexors and extensors in the Tai Chi group was significantly greater than in the sedentary group (p=0.001 to 0.00160°/sec; P=0.002 to 60°/sec extensors; p=0.002 to 120°/sec; 120°/sec flexors, p=0.003). Similarly, there was a significant difference in muscle strength between the Tai Chi group and the walking group (the P values of the flexors and extensors at both speeds were less than 0.001). Conclusion: Tai Chi, as a regular exercise, can increase muscle strength of the general knee flexors and extensors and improve the neuromuscular stability of lower limbs in the elderly. Evidence Level II; Therapeutic Studies - Investigating the result.

RESUMEN Introducción: Los ejercicios pueden retrasar los efectos del envejecimiento y mejorar la función física de las personas mayores. El Tai Chi es una práctica de ejercicio muy popular entre las personas mayores de China. Aunque hay estudios que demuestran los efectos positivos de la práctica del Tai Chi, todavía no hay consenso sobre los estudios comparativos. Objetivo: Evaluar el efecto del ejercicio regular de Tai Chi en la estabilidad neuromuscular de las extremidades inferiores en ancianos, comparando los resultados de los practicantes entre grupos de ancianos sedentarios y caminantes. Métodos: Se seleccionaron 22 practicantes de Tai Chi, con una edad media de 59,3±3,5 años y un tiempo medio de práctica de 18,4±13,2 años. Este experimento pone a prueba principalmente la capacidad de equilibrio en comparación con los individuos que practican caminata y otros sedentarios. Resultados: La fuerza muscular de los flexores y extensores de la rodilla en el grupo de Tai Chi fue significativamente mayor que en el grupo sedentario (p=0,001 a 0,00160°/seg; p=0,002 a 60°/seg extensores; p=0,002 a 120°/seg; 120°/seg flexores, p=0,003). Del mismo modo, hubo una diferencia significativa en la fuerza muscular entre el grupo de Tai Chi y el grupo de caminantes (los valores P de los flexores y extensores a ambas velocidades fueron inferiores a 0,001). Conclusión: Los ejercicios regulares, como el Tai Chi, pueden modificar la fuerza muscular de los flexores y extensores generales de la rodilla y mejorar la estabilidad neuromuscular de los ancianos en las extremidades inferiores. Nivel de evidencia II; Estudios terapéuticos - Investigación de resultados.

Abstract In medical clinical practice, organ transplantation is mainly applied to patients with end-stage organ lesions and organ failure. However, with the development of organ transplantation, many ethical issues and controversies have arisen. From the perspective of bioethics, the article compares the relevant ethical and legal regulations of organ transplantation in various countries. Due to the complexity of the real situation, many ethical dilemmas arise in organ transplantation in China. The article analyzes and researches three aspects of organ donation, distribution, and trading, and finds that there are various ethical problems in these three aspects of organ transplantation in China, such as whether the principle of presumed consent is ethical, whether brain death is legalized, the selection and determination of transplant patients, and whether human organ trading is legalized, etc. With the help of the four principles of bioethics and the current development of organ transplantation in China, the article proposes that organ transplantation in China should follow four ethical principles: the principle of respect for life, the principle of do no harm/benefit, the principle of respect for autonomy, and the principle of justice, in order to provide a defense for the legitimacy of organ transplantation.

Resumen En la práctica clínica médica, el trasplante de órganos se aplica principalmente a los pacientes con lesiones orgánicas en fase terminal y con insuficiencia orgánica. Sin embargo, con el desarrollo del trasplante de órganos, han surgido muchas cuestiones éticas y controversias. Desde la perspectiva de la bioética, el artículo compara las normas éticas y jurídicas pertinentes del trasplante de órganos en varios países. Debido a la complejidad de la situación real, surgen muchos dilemas éticos en el trasplante de órganos en China. El artículo analiza e investiga tres aspectos de la donación, la distribución y el comercio de órganos, y constata que hay varios problemas éticos en estos tres aspectos del trasplante de órganos en China, como si el principio del consentimiento presunto es ético, si la muerte cerebral está legalizada, la selección y determinación de los pacientes de trasplante y si el comercio de órganos humanos está legalizado, etc. Con la ayuda de los cuatro principios de la bioética y el desarrollo actual del trasplante de órganos en China, el artículo propone que el trasplante de órganos en China debe seguir cuatro principios éticos: el principio de respeto a la vida, el principio de no hacer daño/beneficio, el principio de respeto a la autonomía y el principio de justicia, con el fin de proporcionar una defensa de la legitimidad del trasplante de órganos.

Resumo Na prática clínica médica, o transplante de órgãos é principalmente destinado a pacientes em estágio final de lesões e falência dos órgãos. Entretanto, com o desenvolvimento do transplante de órgãos, surgiram muitas questões e controvérsias éticas. O artigo compara, desde uma perspectiva bioética, as regulações éticas e legais relevantes sobre transplantes de órgãos em vários países. Devido à complexidade da situação real, muitos dilemas éticos surgiram no transplante de órgãos na China. O artigo analisa e investiga aspectos de doação, distribuição e comercialização de órgãos, e encontra que há vários problemas éticos nestes três aspectos do transplante de órgãos na China, tais como se o princípio do consentimento presumido é ético, se morte cerebral é legalizada, a seleção e determinação de pacientes que irão receber transplante, se a comercialização de órgãos humanos é legalizada, etc. O artigo propõe, com a ajuda de quatro princípios da bioética e o desenvolvimento atual de transplante de órgãos na China, que o transplante de órgãos na China deve seguir quatro princípios éticos: o princípio do respeito à vida, o princípio de beneficiar/não causar dano, o princípio do respeito pela autonomia e o princípio da justiça, de forma a possibilitar a defesa da legitimidade do transplante de órgãos.

RESUMO Introdução: À medida que a frequência e a intensidade de atividades esportivas escolares continuam a aumentar, lesões acidentais podem ocorrer periodicamente durantes essas atividades. Objetivo: Este artigo discute o papel positivo da ciência e da tecnologia na medicina do esporte ao promover o desenvolvimento de esportes. Concomitantemente, as vantagens da medicina do esporte para as lesões do esporte são analisadas. Métodos: Este artigo utiliza estatísticas matemáticas para compreender a situação atual das lesões por esporte de estudantes universitários e a prevenção e o controle de riscos, e discute o efeito da medicina do esporte nas lesões do esporte. Resultados: Há uma diferença considerável entre a lesão por esporte dos estudantes e o número de vezes, a hora e a quantidade de exercício que eles praticam a cada semana. Quanto mais participam em esportes, menores serão as lesões. Conclusão: O fortalecimento da educação física e da conscientização sobre a prevenção e o controle de risco no esporte na formação dos estudantes pode ajuda-los a evitar lesões no esporte. Nível de evidência II; Estudos terapêuticos – investigação de resultados de tratamento.

ABSTRACT Introduction: As the frequency and intensity of school sports activities continue to increase, accidental injuries occur from time to time during sports activities. Objective: This article discusses the positive role of science and technology in sports medicine in promoting sports development. At the same time, the advantages of medical sports to sports injuries are analyzed. Methods: This article uses mathematical statistics to understand the current situation of college students’ sports injuries and risk prevention and control, and discusses the effect of sports medicine on sports injuries. Results: There is a significant difference between the student's sports injury and the number of times, the time, and the amount of exercise they participate in each week. The longer the participation in sports, the more minor the sports injuries are. Conclusion: Strengthening physical education and strengthening awareness of sports risk prevention and control in student teaching can help students avoid sports injuries. Level of evidence II; Therapeutic studies - investigation of treatment results.

RESUMEN Introducción: En la medida que la frecuencia y la intensidad de actividades deportivas escolares continúan a aumentar, lesiones accidentales pueden ocurrir periódicamente durante esas actividades. Objetivo: Este artículo discute el papel positivo de la ciencia y tecnología en la medicina del deporte al promover el desarrollo de deportes. Simultáneamente, se analizan las ventajas de la medicina del deporte para las lesiones del deporte. Métodos: Este artículo utiliza estadísticas matemáticas para comprender la situación actual de las lesiones por deporte de estudiantes universitarios y la prevención y control de riesgos, y discute el efecto de la medicina del deporte en las lesiones del deporte. Resultados: Hay una diferencia considerable entre la lesión por deporte de los estudiantes y el número de veces, la hora y la cantidad de ejercicio que practican a cada semana. Cuánto más participan en deportes, menores serán las lesiones. Conclusión: El fortalecimiento de la educación física y de la concientización sobre la prevención y el control de riesgo en el deporte en la formación de los estudiantes puede ayudarles a evitar lesiones en el deporte. Nivel de evidencia II; Estudios terapéuticos – investigación de resultados de tratamiento.

RESUMEN Para predecir la capacidad de carga de los recursos hídricos con mayor precisión y orientar mejor las actividades sociales y económicas, se propone un método de predicción de la capacidad de carga de los recursos hídricos regionales basado en una red neuronal artificial. El condado de Zhaozhou se selecciona como el área de investigación de la predicción de la capacidad de carga de los recursos hídricos, y se exploran sus características geográficas naturales, la economía social y la situación de los recursos hídricos. De acuerdo con las características regionales de cantidad y utilización de los recursos hídricos y el énfasis de la evaluación, el sistema de índice de evaluación de la capacidad de carga de los recursos hídricos se construye para evaluar la importancia y el grado de correlación de la capacidad de carga de los recursos hídricos, y el grado de presión de la capacidad de carga de los recursos hídricos se divide en cinco grados. De acuerdo con el estándar de evaluación de la capacidad de carga, se construye el modelo de red neuronal BP de inteligencia artificial. Con base en los principales factores de impacto de la capacidad de carga de los recursos hídricos en esta área, el grado de capacidad de carga de los recursos hídricos se obtiene mediante el cálculo del peso y la iteración de convergencia utilizando el modelo de red neuronal y los datos de factores de influencia, para realizar la predicción de la capacidad de carga de los recursos hídricos. Los resultados de la investigación muestran que el modelo de red puede satisfacer la demanda de precisión y los resultados de la predicción tienen un alto grado de ajuste con los datos reales, lo que indica que la inteligencia humana puede obtener resultados de predicción precisos en el proceso de predicción de la capacidad de carga de los recursos hídricos.

ABSTRACT To better predict the water resources carrying capacity and guide the social and economic activities, a prediction method of regional water resources carrying capacity is proposed based on an artificial neural network. Zhaozhou County is selected as the research area of water resources carrying capacity prediction, and its natural geographical characteristics, social economy, and water resources situation are explored. According to the regional water resources quantity and utilization characteristics and evaluation emphasis, the evaluation index system of water resources carrying capacity is constructed to evaluate the importance and correlation of water resource carrying capacity. The pressure degree of water resources carrying capacity is divided into five grades. According to the evaluation standard of bearing capacity, the artificial intelligence BP neural network model is constructed. Based on the main impact factors of water resources carrying capacity in this area, the water resources carrying capacity grade is obtained by weight calculation and convergence iteration by using neural network model and influence factor data to realize the prediction of water resources carrying capacity. The research results show that the network model can meet the demand for precision. The prediction results have a high degree of fit with the actual data, indicating that human intelligence can obtain accurate prediction results in water resources carrying capacity prediction.

Abstract Anthocyanin plays an important role in wine color and quality. The anthocyanin concentration in grapes depends on the variety and is influenced by environmental factors, such as temperature, sunlight, soil, and grapevine practice. In this study, the anthocyanin profiles of two varieties, namely Cabernet Sauvignon and Merlot, from five regions (11 plots) in Xinjiang were examined. The climatic parameters performed noticeable difference between northern and southern of Xinjiang region. Result showed that northern of Xinjiang possessed the relatively lower humidity and higher average temperature in comparison with southern area. In addition, the total anthocyanin profiles of Cabernet sauvignon, which in Changji, Wushitala, Heshuo respectively, were higher than of other plots. This result might be caused by which those have relatively higher altitude than in other plots. For merlot, the higher anthocyanin concentrations performed in southern of Xinjiang, which could be contributed to the lower average temperature and higher relative humidity. Comparing Cabernet sauvignon and Merlot, the latter was easily affected by environment factors, and the anthocyanin composition and concentration of Merlot showed significant differences in different regions.

OBJECTIVES: Here, we aimed to compare the clinical effects of mycophenolate mofetil combined with either tacrolimus or with cyclophosphamide on lupus nephritis (LN) and to analyze their influence on the expression of cystatin C and on transforming growth factor-1 (TGF-β1). METHODS: A total of 234 patients were randomly divided into two groups: group A, for mycophenolate mofetil combined with tacrolimus (n=117) and group B, for mycophenolate mofetil combined with cyclophosphamide (n=117). The enzyme-linked immunosorbent assay was adopted to detect the expression levels of serum TGF-β1 and cystatin C before and after treatment. RESULTS: The total effectiveness rate in group A was much higher than that in group B. The times of effectiveness and effect validity in group A were much lower than those in group B. The expression levels of serum TGF-β1 and cystatin C decreased slightly after treatment in the two groups, and those of group A were much lower than those of group B. CONCLUSIONS: The combination of mycophenolate mofetil and tacrolimus showed better clinical efficacy on LN and was safer than that of mycophenolate mofetil and cyclophosphamide. Moreover, the drug combination of mycophenolate mofetil and tacrolimus greatly reduced the expression levels of serum TGF-β1 and cystatin C.

Abstract A comprehensive investigation was conducted to determine the effects of exogenous melatonin treatment of pre-veraison grapes on phenolic components and antioxidants of grape berries and wine. The results showed an increase in the concentration of cyanin-3-O-glucoside, peonidin derivatives, and two flavanols [namely (+)-catechins and (−)-epicatechins] of grapes, as well as flavonols in both grapes and wine due to the application of melatonin. These compounds are derivatives of the catalysate of flavonoid 3’-hydroxylase (F3’H) in the flavonoid synthesis pathway of plants. It can be inferred that melatonin increased the activity of F3’H or expression of VvF3’H during grape berry maturation. In addition, two melatonin treatments of pre-veraison grape berries reduced the DPPH radical-scavenging ability and copper ion-reducing power of grapes and wine, whereas one melatonin treatment just reduced those in wine. This study will provide a practical strategy to change the phenolic composition of grapes and wine.

Abstract Rabbit with hypercholesterolaemia is an important model for studying cholesterol metabolism disease. This study aimed to evaluate the expression stability of nine reference genes for quantitative PCR (qPCR) analysis in adrenal gland, liver, spleen, and kidney tissue from rabbits with hypercholesterolaemia. In total, 30 male Harbin Large White (HLW) rabbits were fed a normal feed (n = 15) or a high cholesterol feed (n = 15) for 8 weeks to induce hypercholesterolaemia. Nine reference genes were verified by qPCR using cDNA extracted from rabbit tissue samples. For qPCR analysis, reference genes were evaluated using the RefFinder and GeNorm algorithms. Overall, seven rabbits with hypercholesterolaemia were identified based on body weight and total cholesterol measurements. Combining the results of the RefFinder and GeNorm algorithms, the most stable reference genes were hypoxanthine phosphoribosyltransferase 1 (Hprt1) and eukaryotic translation elongation factor 1 alpha 1 (Eef1a1) in the adrenal gland, β-2-microglobulin (B2m) and glyceraldehyde-3-phosphate dehydrogenase (Gapdh) in the liver, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta (Ywhaz) and Gapdh in the spleen, and peptidylprolyl isomerase (Ppia), β-actin (Actb), succinate dehydrogenase complex subunit A flavoprotein (Sdha), and B2m in the kidney. Taken together, our results confirmed that Hprt1 and Eef1a1, B2m and Gapdh, Ywhaz and Gapdh, and Ppia, Actb, Sdha, and B2m were the best reference genes for qPCR analyses in adrenal gland, liver, spleen, and kidney tissue, respectively, of rabbits with hypercholesterolaemia.

Abstract Rain-shelter cultivation has been proven an important cultivation method for grape-plantings in continental monsoon climate zones, of which white plastic films are the most common shelter material. However, while this method and material reduces the occurrence of the disease, it can also decrease the grape berry quality. Five colours (including red, yellow, blue, purple, and white) of plastic films were covered above Cabernet Sauvignon (Vitis vinifera L.) grapevine rows before veraison. Rain-shelter cultivation reduced air temperature, wind speed, and total solar radiation and enhanced relative humidity in the fruit sphere of grapevines. For each particular colour plastic film, the irradiance of its corresponding spectrum band in the canopy of vines was higher than with other colour plastic films. Meanwhile, the blue plastic film treatment significantly promoted the accumulation of total phenolics, anthocyanins, flavonoids, tannins, and phenolic acids more than the other colours of plastic films. Blue plastic films are more beneficial for berry quality promotion of wine grapes, especially Cabernet Sauvignon, under rain-shelter cultivation in continental monsoon climate zones.

Summary Objective: To investigate the changes in serum cardiac myosin light chain 1 (CMLC-1) levels in children with fulminant myocarditis (FM) during continuous blood purification (CBP), as well as to analyze its correlation with other laboratory indexes. Method: Twenty-four (24) children with FM who underwent CBP were enrolled. Before and during treatment (48 and 72 hours after treatment, or death), the optical density value of serum CMLC-1 was measured using enzyme-linked immunosorbent assay, and then the serum CMLC-1 concentration was calculated. The correlations between CMLC-1 OD value change and laboratory indexes including creatine kinase-MB (CK-MB), troponin, myohemoglobin and N-terminal pro-brain natriuretic peptide (NT-proBNP) were analyzed. Results: The serum CMLC-1 concentration significantly increased in the children with FM and decreased obviously during CBP therapy. In the same period, the change of CMLC-1 concentration were positively correlated with creatine kinase-MB (r=0.528), troponin (r=0.726), myohemoglobin (r=0.702), and NT-proBNP levels (r=0.589). Conclusion: The serum CMLC-1 concentration increases significantly in children with FM, but CBP therapy can effectively control this increase.

The aroma characteristics of wines from four Vitis vinifera grape varieties (‘Cabernet sauvignon’, ‘Merlot’, ‘Chardonnay’, and ‘Italian Riesling’) grown in three shoot positions were evaluated by HS-SPME-GC/MS. In this study, the numerous significant differences found in most of the aromatic compounds influence of different shoot positions on the quality of wine. The results showed that the middle shoot position increased significantly the aroma concentration in the majority of wines investigated. The volatile components showing the greatest differences in the wines of different cultivars were aldehydes and terpenes. 8 and 11 compounds were found and quantified (OAVs>1) in the two red wines and white wines at concentrations higher than their corresponding odor thresholds, respectively; and therefore they significantly contributed to the wine aromas. According to their OAVs, fruity, floral, cheese and fatty aroma strongly influenced the characteristics of the four monovarietal wines, while the two white wines showed the green and fresh aroma characteristics. These results are related to the different microclimate of the canopies of the different shoot positions and varieties. They suggest that proper elevating the fruiting zones could improve the accumulation of aroma compounds in wines from the different varieties. On the other hand, grapevines trained to systems with uniform fruiting zones could improve the quality of wine.

The microwave-assisted extraction conditions of flavonoids in Physalis alkekengi. var. franchetii stems were optimized using a L3³ Box-Behnken Design. The optimized extraction conditions were determined as follows: 60% for the ethanol concentration, 12.4 for the liquid-to-solid ratio, and 531.4 W for the microwave power, respectively. The yield of the extract obtained under the optimized conditions was 3.85 mg g-1 which was close to the predicted value. In addition, the extract exhibited potent acetylcholinesterase inhibitory activity, with the IC50 value of 11.61 μg mL-1 and a maximal inhibition ratio 89.81%. Both the yield and activity were better than reflux extraction.

En esta investigación se optimizaron las condiciones de extracción de flavonoides a partir de Physalis alkekengi var. Franchetii, asistida por microondas, usando un diseño L3³ Box-Behenke. Las condiciones determinadas fueron etanol 60%, la relación líquido:sólido fue de 12.4 y se usaron 531.4 W para el microondas. El rendimiento obtenido fue de 3.85 mg g-1, el cual es cercano al valor predicho. El extracto obtenido inhibe de forma importante la actividad de la acetilcoliestearasa, con una IC50 de 11.61 µg mL-1 y una inhibición máxima de 89.91%. Estos resultados son mejores que los obtenidos con el compuesto obtenido mediante extracción por reflujo.

The corridor area of Gansu Province is one of the most important wine grape growing regions in China, and this strip of land results in a significant difference in terms of terroir between its regions. Chemical composition and antioxidant capacity of the main wine grape varieties (Vitis vinifera L.) cultivated in the corridor area of Gansu Province in northwest China were compared. Three regions (Zhangye, Wuwei, and Jiayuguan) were selected to explain the influence of soil and climate conditions on the quality of wine grapes. This study aims to investigate the effect of different regions on berry composition and antioxidant capacity, providing a general evaluation of red and white wine grapes quality in the corridor area of China. The results showed that ‘Merlot’ grapes grown in Zhangye had the best quality among the different varieties in the three regions of Gansu evaluated. The moderate temperature and nitrogen deficiency were associated with improved fruit quality. It was identified that the most suitable grape variety from Zhangye is ‘Merlot’, and that ‘Cabernet Sauvignon’ and ‘Italian Resling’ are the most suitable varieties from Wuwei and Jiayuguan, respectively.

In this study we focused on the development of Cabernet Sauvignon grapes and investigated changes in the activity of alcohol dehydrogenase (ADH) and hydroperoxide lyase (HPL) in different tissues. We sampled grape skin at four, six, seven, eight, nine, 10, 12, 14 and 16 weeks after anthesis; developing flowers when blooming at 0%, 5%, 50%, and 90%; and leaves at two and four weeks before anthesis and at two, four, six, eight, nine, and 10 weeks after anthesis. We also examined the type and fluctuation of volatile contents. ADH activity increased with the development of flowers and grape skins, which led to the increasing of types and concentration of alcohols. Low levels of 9-HPL led to low concentrations of C9 compounds. According to this paper, C6 compounds became abundant with the development of grape berries, while the activity of 13-HPL kept at a low level in the flowers and grape skins. There might have been a high level of 13-HPL activity from the end of flowering until fruit setting that we did not detect. Furthermore, similar C6 and C5 compounds were detected across all tissues, including hexanal, (E)-2-hexenal, (Z)-3-hexenal, (Z)-2-penten-1-ol, (Z)-3-hexen-1-ol, 1-hexanol and 3-hexen-1-ol. Generally speaking, the concentrations of C6 and C5 compounds could be used as the criterion of maturation of the three grape tissues.